solubilization buffer (Thermo Fisher)

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Thermo Fisher solubilization buffer
Solubilization Buffer, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/solubilization+buffer/HEPES/pmc13059800-318-6-22
Average 99 stars, based on 1 article reviews

solubilization buffer - by Bioz Stars, 2026-09

99/100 stars

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Protein Concentration:

Article Title: Single-Molecule Structures and Kinetics of the AAA-ATPase Bcs1 translocating folded ISP.
Article Snippet: Pan et al. 2024 Transport kinetics of ISP and Bcs1 Version: October 19, 2025 2 Bcs1 is a AAA-ATPase that transports the Rieske iron-sulfur protein (ISP) across the inner mitochondrial membrane.. Bcs1 is a particular molecular machine in many regards: In contrast to canonical, hexameric, soluble, AAA-ATPases (i) Bcs1 is heptameric, (ii) Bcs1 is a transmembrane protein with each subunit featuring a transmembrane helix, (iii) Bcs1 transports ISP in its folded state, and (iv) Bcs1 works using a concerted mechanism and not a hand-overhand or stochastic mechanism.. How Bcs1 binds and transports folded ISP is unknown.

Acid Assay:

Membrane:

Article Title: A lipid plug affects K 2P 6.1(TWIK-2) function
Article Snippet: .. Using a 16 G Precision Glide needle and 30 mL syringe, the membrane pellet was homogenized in 200 mL solubilization buffer (buffer S) (100 mM KCl, 50 mM Tris-HCl pH 8.0, supplemented with 1 mM PMSF, 0.1 mg mL -1 DNase1, 1 % (w/v) n-Dodecyl-β-D-Maltopyranoside (β-DDM), and Pierce protease inhibitor. ..

Article Title: A tetrameric SpoVA2 membrane complex is required for DPA transport into Bacillus anthracis spores
Article Snippet: .. The membrane pellet was homogenized in solubilization buffer (20 mM HEPES at pH 7.6, 150 mM NaCl, 20% glycerol, 1% n-dodecyl-β-D-maltopyranoside [DDM; Thermo Fisher]) and rotated for 1 h at 4°C. ..

Article Title: Supporting Information for Affinity Selection of Double-Click Triazole Libraries for Rapid Discovery of Allosteric Modulators for GLP-1 Receptor
Article Snippet: .. The GLP-1R TMD was extracted from the membrane by adding a 2× solubilization buffer [1 M NaCl, 100 mM HEPEs (pH 7.5), 1% (w/v) n-dodecyl-beta-D-maltopyranoside (DDM, Affymetrix) and 0.2% (w/v) cholesteryl hemisuccinate (CHS, Sigma)] and stirring for 3 h at 4 °C. .. Then the solubilized mixture was ultra-centrifuged at 160,000 g for 30 min, and the supernatant was incubated with TALON IMAC resin (Clontech) at 4°C, overnight.

Protease Inhibitor:

Article Title: Structural basis for malate-driven, pore lipid-regulated activation of the Arabidopsis vacuolar anion channel ALMT9
Article Snippet: .. Harvested cells or pellets were resuspended sequentially in hypotonic buffer [20 mM HEPES pH 7.6, 1 mM Tris (2-carboxyethyl) phosphine (TCEP), 0.25x PierceTM EDTA-free protease inhibitor (Thermo Fisher Scientific)], hypertonic buffer [50 mM HEPES pH 7.6, 1 M NaCl, 1 mM TCEP, 5 % (v/v) glycerol, and 0.25x PierceTM EDTA-free protease inhibitor (Thermo Fisher Scientific)] and solubilization buffer [50 mM HEPES pH 7.6, 150 mM NaCl, 20 mM imidazole pH 8.0, 1 mM TCEP, 5 % (v/v) glycerol, 0.5x PierceTM EDTA-free protease inhibitor (Thermo Fisher Scientific), 2 % (w/v) n -dodecyl β- d -maltoside (DDM), with or without 0.2 % (w/v) cholesteryl hemisuccinate (CHS), 2 mM MgCl 2 , and 12.5 U mL −1 benzonase]. ..

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